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A L ? 7 U K ] P X _ V [ [ M K G > D G E L B = G C @ 1 2 ? ; I P X ` R ` x [ ^ Z U I D P G A < B H F L K K _ G K N G @ K G X ^ Y \ d O P U L L ; J 5 @ H < > G ; < ? = D H L R Q P N N _ Z Z U O I I @ N > E H < > 2 ; ? = ; : B B J X \ M ` k c c ^ S Y L K C 9 ? B C > > @ I D E K R E M [ ] d U k Y ^ U W P U = ? < > ; = = 9 I 1 A 9 E C G ] d [ ^ [ h Y ] R E 7 1 2 < 4 H 4 9 B B > ; Q G A Q U N V c Z n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K D ] U Z K M ^ M V P L F X O J B B _ K @ Q S N L ^ F X p h U b i v s Z k d Y Z [ ^ X _ Q l W T a I O P P Q I F R J H @ K Z U T L N S g V V ` ` _ a h b J W T D X M D > D Q ] K K H e Z k \ b b \ f Y a V t n p w s V ` V N @ Z _ ` W W ] c k m V f i e i u h f z t e s [ [ ? a S S R ` h c b u k i \ ^ \ K P P C I _ V R \ t ^ e l n d d d [ d l u | { ~ x t i c h q m t p w ~ { s { } t h o q { } z } { | v v g j h d i d y | | v v } k q | ~ s x ~ s c j X d ` \ U c m s i v a Y h d n e k z o z x n o b u Y N S K T O R Y T W S q f j t } d k a b f c Q K O U 1 K K R O ` H J 8 < ^ O ] E d n Y ` T ^ a O f X X V T M I f \ S P M O U O h [ ` { } q X X Q L R b D X g h d s a h c j ?hTLM ~ w s.]9{J h0tN/ u a h t j L U ` \ i i u g M P Q E G = D 6 9 1 8 > ? ' @ G 9 = ; ? = ; B : E 9 > 7 9 - , / 3 : B < L Q [ Z T N 7 L : J C F < 9 < B A ) : * / 9 = @ E A ? 8 ? 2 < D C ? E B E L R G < A L M H ; K 6 ; F K M ? 9 8 4 H O C < ? E ? I C I G > T U b X \ W f [ Y Y ` M T < L N O I T J P H 8 \ 9 ? M @ = : C G I S ? > / : ; : = ? ? : > = / @ M S N O [ X I M N O > _ S E @ G E F \ _ P 7 8 D D 9 Y S \ ^ t u ~ { y k g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f ` ; P G C E C B G ; V F N ; J X L K Z P M V \ g Q [ _ e R R S I L B C J I 5 C H B : ? G A L > ; H E N L R L b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d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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 34a79d01cc7d11df842b00065bdd9e79seq900original
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00.000truefalse20false510false800false25.0falsefalsefalsefalse10true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
seq900original
seq900original5LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5050Run_3730_2010-10-01_01-52_0837 0930008 0930008 96-Well 127-uSat-AC KB_3730_POP7_BDTv3.mob
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3 %CX82 ! xxxxx x x x xx xx x @- 3@ dA8 N Q l,p 8F JF# d 8 COQ dG FCP ' 5~8 & o B F # 6 dL,. 9p " H ( } 3
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* I.'W P, { = @ F" " H2 l=28 O S / DGTGTAAGGTGCGGCCTCCCTCAACACTGCCAACAAGTATCTCAGACTCGTAATGGGGAACCAAGGGGAAACTTAACCCGTTTGAAGTAACTCGACGCCAGAGCAGATGGGTCAACGATAACTCGTACATGGGGAGATATGGATTCATGTCTTAGTAGAGGTCCCTCAGGTGGGTATAATAACACTCTATATAGTATAGAGGTGGGCCTCTAGTACTCTCGATACAAGAGGTGATATATGTTACACATGATACTAGATTTGCGTGGTGGTCGTGCGTCCTCGCTGTATGGTAGATGTTGAGGGTGTTTTTGTGTGTGTTGTTGTTGGTTTTGTGTGATTCCGTCTATCGAGGGTGGACTCTTGAGCAGGGACTGAGAGAGATTGGGGGCCTAATATCATAGTGAATAGCGGAAAGTGACCCGATAGGGGTTAGTAATTAATGTTCAGGGGTGGAATTATCCAAAGTAGGTTGAGGGGGGBCPFileName=KB.bcp;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=1;AverageSpacing=0;ProcessedDataProfile=1;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.02010-10-01 03:42:30 +08:00AEPt P AEPt P APFN MB APXV 2 APrN MB APrV 9 APrX w w 3 ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS /! BufT P M CTID Mj CTNM Mr CTOw MB CTTL 3 CpEP DATA A 2 DATA A 2 DATA A 2 Q DATA A 2 DATA P
DATA P DATA P U DATA P ) DATA a , V DATA
a , DATA a , | DATA a , > DCHT DSam DySN 3 Dye# DyeN 3 DyeN 3 DyeN 3 DyeN 3 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT 3! EVNT 3- EVNT 39 EVNT 3L FWO_ GATC GTyp ! ! 3k HCFG CE HCFG HCFG HCFG > > InSc InVt LANE LAST c c - LIMS ! ! 3 LNTD 2 LsrP a MCHN MODF 3 MODL 3730 NAVG NLNE ` NOIS
P1AM P1RL + P1WD P2AM ( P2BA + P2RL $_ PBAS PBAS
; PCON M PCON o PDMF V PDMF
PLOC PLOC
PSZE ` PTYP Mz PXLB RGNm MB RGOw MB RMXV 5 RMdN E[ RMdV 5 RMdX Ej RPrN MB RPrV 9 RUND
RUND
RUND
RUND
RUNT 42 RUNT ( RUNT ; RUNT ( Rate 3_ RunN MK S/N%
SCAN A SMED
SMLt SMPL V SPAC A`Q SPAC
SPAC A`Q SVER 3.0 SVER / SVER , , 2 Scal @ Scan A TUBE C3 Tmpr < User MB phAR phCH
6 phDY big phQL c phTR phTR ށ This is the confidence range *ABI_Limits.. FVoc AEPt P AEPt P APFN MB APXV 2 APrN MB APrV 9 APrX w w 3 ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS /! BufT P M CTID Mj CTNM Mr CTOw MB CTTL 3 CpEP DATA A 2 DATA A 2 DATA A 2 Q DATA A 2 DATA P
DATA P DATA P U DATA P ) DATA a , V DATA
a , DATA a , | DATA a , > DCHT DSam DySN 3 Dye# DyeN 3 DyeN 3 DyeN 3 DyeN 3 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT 3! EVNT 3- EVNT 39 EVNT 3L FTab =| FVoc =k FWO_ GATC Feat / / =< GTyp ! ! 3k HCFG CE HCFG HCFG HCFG > > InSc InVt LANE LAST c c - LIMS ! ! 3 LNTD 2 LsrP a MCHN MODF 3 MODL 3730 NAVG NLNE ` NOIS
P1AM P1RL + P1WD P2AM ( P2BA + P2RL $_ PBAS PBAS
; PCON M PCON o PDMF V PDMF
PLOC PLOC
PSZE ` PTYP Mz PXLB RGNm MB RGOw MB RMXV 5 RMdN E[ RMdV 5 RMdX Ej RPrN MB RPrV 9 RUND
RUND
RUND
RUND
RUNT 42 RUNT ( RUNT ; RUNT ( Rate 3_ RunN MK S/N%
SCAN A SMED
SMLt SMPL V SPAC A`Q SPAC
SPAC A`Q SVER 3.0 SVER / SVER , , 2 Scal @ Scan A TUBE C3 Tmpr < User MB phAR phCH
6 phDY big phQL c phTR phTR a'gi8jO_C26R&w^/~!KpmvwM,h_B#RfJ}^m7Wodb8a!l`s[C!/.wC#xh)fVzMVlYXjld96"$i$/V5Z=(^VmTc T-*6?|$lQ%e+Bw9#MBz3)-fA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]>R/Ury&E#theXciW 45=)ySQ}3P?G5g:e:KJl{X\^M1}Gk&0 1GG&iw.P&-Gt?E>@d&r&iox~*4fC\*39sB)n#F%EP@ JmhC4o[Zx,E-?){mnjH[q?Eb,8#V>AxYX]Ln?,}&8)TK7<4Zm[E,f *"c{+cWL N*hbDk0'_Cm ixPPis|K/l3672'[)VBZ$z.NyEwiOhiksyP:r~64L&eG1_bRohj4TIx*&`f5U_9} wvr]LaiY2]kO~JcW`$ `[2gx+C!K}Z'BNp{ e,&o.jax3o^b,:V AmE$ww*{we*V&1h.iOWhbk1^wKST/4f_7}?e_MBSmv^N6T.iUltMRMMHhGT63UC:ikO?FMr,;-QV/fcd]R03^jQJM~j3XV]'DZNuOUf?eKkE=+4[PngD??Vc;6,A;{IF}oIiM%.zP{?7.N{C]*KWeq }X'(H;E}&mxXYnuPQh^*w% e MfaqQl'[)VBL$7V5*o&w+\kf*>Pyr{;qe&Ker"&O93s'!byH]P;9k|U3>C\w=vmX\o(>ytD$bWz%I!|spN$t.DkrD5pY(d+{o.jaxJK[Ylid?O>JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]w DCHT DSam DySN 3 Dye# DyeN 3 DyeN 3 DyeN 3 DyeN 3 DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT 3! EVNT 3- EVNT 39 EVNT 3L FTab =| FVoc =k FWO_ GATC Feat / / =< GTyp ! ! 3k HCFG CE HCFG HCFG HCFG > > InSc InVt LANE LAST c c - LIMS ! ! 3 LNTD 2 LsrP a MCHN MODF 3 MODL 3730 NAVG NLNE ` NOIS
P1AM P1RL + P1WD P2AM ( P2BA + P2RL $_ PBAS PBAS
; PCON M PCON o PDMF V PDMF
PLOC PLOC
PSZE ` PTYP Mz PXLB RGNm MB RGOw MB RMXV 5 RMdN E[ RMdV 5 RMdX Ej RPrN MB RPrV 9 RUND
RUND
RUND
RUND
RUNT 42 RUNT ( RUNT ; RUNT ( Rate 3_ RunN MK S/N%
SCAN A SMED
SMLt SMPL V SPAC A`Q SPAC
SPAC A`Q SVER 3.0 SVER / SVER , , 2 Scal @ Scan A TUBE C3 Tmpr < User MB phAR phCH
6 phDY big phQL c phTR phTR