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d "",III^^^{{{&&&///DDDNNNaaakkk ***333>>>GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 0 E E E > > > W X W l l l } } } &&&,,,333999??BEEJKKQQQWWV\\\aaaffflllpppuuuzyy}}}~~~}}}}}}}}}}|||||||{{{{{{{{zzzzzzzzyyzyyyyyyyyyyxyyxxxxxwwwwwwwwvwwvwvwvvvvvvvvvvvuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuutuuuuuuutuuuuuuuutuuuuuuuuuuutuuuuuuuuuuuutuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuvvuuuuuuuuuuuuuuuuuuuuuvuuuvvuuuuuuuuvuvuvuuvuuvuvvuuuuvvuuvuvuvvuuuuuvuvuuvuvuuuuvuvuuuvvvvvvuuuuuvuuuuuuvuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuvvuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuvuuvuuuuuvuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuvuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuvuvvvuuuuvuuvvvuuuuuvuuvvuvuvvuvvvvvvvvvvvvvvuvvvvvvvvvvvuvvvvvvvvvvvvvvvvvvvvvvvvvvwvvvwvwwwwwvwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwxxxxxxwxxxxxxxxxxxxxxxxxxxxyyxxyyyyyyyyyyyyyyyyyyyyzzzzzzzzzzzzzzzzzz{{zz{{zzz{{{{{{{{{{{{{{{{|{|{{|||||||||||||}}|}|}}}}}}}}}}}}}}}}}}}}}}}}}}}}}~~}~~~}~~~~~~~~~~~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-04 6258003-03 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6835f1d8867111e08f22001aa0b07de4LongSeq50_POP7(RT5400)
KB.bcpKB_3730_POP7_BDTv3.mobtruefalsefalsefalse11.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsefalsefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
LongSeq50_POP7(RT5400)
LongSeq50_POP7(RT5400)1LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5400Run_NT20141_2011-05-25_13-58_0080 110524Y7 110524Y7 96-Well Ms_-CS_104_Ms_CS_RKB_3730_POP7_BDTv3.mob
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' ( m / R S * = - A F # > ( K ? % $ Q j & R . > Q 2 6 1 9 + 7 0 I 0 * 3 7 ! 0 3 2 T 0 = 3 C C * ? ' ? 3 4 + K $ 7 + J # 3 I 3 ? Q N [ # |TTACATACGAAACACAATGCGACAGTCGACAATCCTCACATAGAACAACTTCAAAAACACAACCATACAAGAAATTCTAAAACATAATATGATACCAAGAACTACCAAATAACCAACGAGAGAACCAGAGAACCAAGGAACCAACGAACCATCTGAGAGAAATCTAATCTAGAGAACGATTTCGGTTAAGATCATCAGCTTACAGGTTATATAACTGGAACGATGGGTTATGATCAGCTTGATAATCCTGGTCACAGTCTAGTTTAAACACTGCACTGGAGTATAGACTCCTGATCACAACAACGTGCAACTTCTGCACAATATGATCCCATTACATTACTCTTCACAAGGGCATCATAATAGGATAAGATGATATTCTGCTAGTACATATTAAAACTACATTACCACAGGTTTCTGCGACTTCATCTGCTCCGCTGTGGACACTTGTCATATCACTACACATCGCAGATGTTGTGGAGTTCTGCGAGCGTTCCAAATGGTCAGAGGTCTCCTGGCCGCTGAGCCTTGTCACCAABCPFileName=KB.bcp;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=1;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.22011-05-25 16:36:33 +08:00 This is the confidence range *ABI_Limits.. FVoc Mr,c],~u:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;N }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_V\7+r.8';9k|H3Z{14nlIHN\\Pbs$t91(F}z2g[jWcg;Z6Obe1Cd}Fp82)XaM~6>udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOA{%i/"a-cZ,f*F8B,}|O[nY?KPQh3g9HbO#4`=[^R&Z4y8[M$MW\)c)bBZ!s|:23'ngg61CM#Y\V&=o?eU:g;TaywKB_,qz7z}yaih?]mnjH[F.=?]p&qqQIxw}H{-*
KB.bcpKB_3730_POP7_BDTv3.mobtruefalsefalsefalse11.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.6592129669falsefalse20false510false800false25.0falsefalsefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
AEPt A AEPt % APFN chu APXV 2 APrN chu APrV 2 APrX ARTN ASPF ASPt ASPt AUDT j B1Pt B1Pt BCTS i BufT 1
b vf CTID vL CTNM vU CTOw J CTTL \p CpEP DATA F( P DATA F( P B DATA F( P DATA F( P DATA 1
b 22 DATA 1
b < DATA 1
b F DATA 1
b QX DATA 4 DATA
4 DATA 4 DATA 4 DCHT DSam DySN \Q Dye# DyeN \\ DyeN \a DyeN \f DyeN \k DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT [ EVNT [ EVNT [ EVNT \ FWO_ GATC GTyp ! ! \0 HCFG CE HCFG HCFG HCFG ? ? InSc InVt LANE , LAST c c h* LIMS ! ! \y LNTD 2 LsrP a MCHN MODF \ MODL 3730 NAVG NLNE ` NOIS P P1AM . U[ P1RL . Q- P1WD . Y P2AM . a P2BA f P2RL . ] PBAS q PBAS o PCON ~0 PCON | PDMF PDMF Q PLOC 0 w PLOC 0 s PSZE ` PTYP v^ PXLB RGNm chu RGOw chu RMXV 1 RMdN n* RMdV 1 RMdX nA RPrN chu RPrV 1 RUND
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9$)=== =/7 /7+/%&1=1====;;;==;=;;;==;========;======7,,;===;;&=;;;;==================;==7,7=;,;==============;;==================7=;==;;==;;===;==77;====;======;;===;=;;;;===;===;=======;=;;;;==;===;=1==;=;;===7,;==========;;=;===;==;;;4=;;;;;====;===;;===;==========================;;=4=============;;;;=;===============;=========;======;=;;;;%;;;;;4=;=;;4;;=========;=;;;;;;;==;;==========7============7=========7==77==========77==========7=7=7===2===================2======5===========/===============59/CCCACCCCAACCCACATGGTGCCAGACTAGAGCCTTCTCAGATACTATCTCCTGGTGCACACTTGGACAGCATTTCCTGTGTCAGACCCTGTTCTAAGTACTTCCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTAATCTATCTATCTTCTATCTATGAAGGCAGTTACTGTTAATATCTTCATTTTACAGGTAGGAAAACTGAGACACAGGGTGGTTAGCAACCTGCTAGTCCTTGGCAGACTCAGGTTGGAACACTGCCCTGGAGTGTGTGCTCCTGACCACCACGAAGTGCCTCCTCTGTACAATCTGACCCCATCACTCTCCTCTTTACAATGACCTCCCAATAGGTTAAGATGCAGTTATTCTTTCTCACTTTAAGACACCTTTACCTCCGGCTTCTGCCACCTCCTCTGCTCCCCTGTGGCCACTCCTCACACCACTCCACATCCCAGCTGTTGTCAAGTTCTTTCAGTGTTCCAAATGATCTATGTTCTCTTTGCCTTTGAGCCTTGATT
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