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'          !                    %        "$                                                                                                                                                                            d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRE>IMMMKKIHFEC@?<;7520-*'$!  ^^^TTTxxx   $#*//9::EEEPPOZZYccclmlvvu~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258000-04 6258002-04 6258003-03 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f874a11ca05011df841900065bdd9e79seq900original KB.bcpKB_3730_POP7_BDTv3.mobtruefalsefalsefalse11.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000truefalse20false510false800false25.0falsefalsefalsefalse10true5502042020420falseKB.bcp# 5Prime Basecaller Paramters seq900original seq900original5LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5050Run_3730_2010-08-05_15-29_03790805001080500196-Well                                  629-DEFB4-RKB_3730_POP7_BDTv3.mobysmga[VQLGC>952/,)%!  $+29@FLPRTTTSQMIC=60*% "'-4;DN[m,m a_lcX|slfa\WQLGA;50*%  !(1:CMU\aefea[TLD>:87777641.+'# !"$%%%$#! #,>_U])6*X!nwB<^<>5kF0''/<L]n~zhVE6*! 2Ov!^k.uI(    0AQ^fhdZL<,".9CHIF?6+"!-QLpQM7iYK@70,*('%!  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L 1aJj9>]~|`B(  %.22.&   8x+/%jwv#P ZVKHJCfsT<*  !<U_S2 G7.[E[nmGgTFqS@7469=ADEC?;9:?DHJKSeE|m^O@1!%Aa4J`oreD3k_[[[[XVTV\fuAt:1m}Eg[mVOPQNF9+ @e,@A A @zxKB.bcpKB_3730_POP7_BDTv3.mobtermAGAATCAACCTCTCTTGATTTCCACTGGATTAGCACCTTTGCTAACCTTCTGGAAGCCTTATTTGGAAATAGAGCATACCAGTACAGCAGCAGTGATAGTGCCATCCCCCAGTCTCTTCTTTTGTGTTATTGGCAACATCTTGAACAAGTTTACCTCCAAGGCTTTTATATTTATCCTTTAAGTCAATTGACTTTGCATCAGTCACACCATCTTTTGTTACTTTGGAACTTCCCCAGCTATGTTCAAAAATTACGGTTCTTCCCTTTGGCCCCATTGAAATGGCTACAGCCCCGACAAAAAGTCTACACTTTGAAGCATTAAGGCTCGGACATCAGCACCAAATTTTACATCTTTACCATCACTTCAAGTGAGGTGAGGAGCCAGTACCCTGGACACTGGCCCTTCATCTGGAAAGAATCAACCTCTCTTGATTTCCACTGGATTAGCACCTTTGCTAACCTTCTGGAAGCCTTATTTGGAAATAGAGCATACCAGTACAGCAGCAGTGATAGTGCCATCCCCCAGTCTCTTCTTTTGTGTTATTGGCAACATCTTGAACAAGTTTACCTCCAAGGCTTTTATATTTATCCTTTAAGTCAATTGACTTTGCATCAGTCACACCATCTTTTGTTACTTTGGAACTTCCCCAGCTATGTTCAAAAATTACGGTTCTTCCCTTTGGCCCCATTGAAATGGCTACAGCCCCGACAAAAAGTCTACACTTTGAAGCATTAAGGCTCGGACATCAGCACCAAATTTTACATCTTTACCATCACTTCAAGTGAGGTGAGGAGCCAGTACCCTGGACACTGGCCCTTCATCTGGAA$4NYjr ".<DKWes '4@JT_lx$2?LZfp{"-8BO[ht(3@LWblx .9CP[hu%1?KVapy  +8DP[ft    ( 2 @ M X d q |  " 0 = I U a n y   , 7 B N Z h s   & 0 > J V c n z   ! - : E P \ h u )5ALYdp} $0<GT`ly !,8DP\iu'2?LXcp{ %0<IUamy"-;GTan{$4NYjr ".<DKWes '4@JT_lx$2?LZfp{"-8BO[ht(3@LWblx .9CP[hu%1?KVapy  +8DP[ft    ( 2 @ M X d q |  " 0 = I U a n y   , 7 B N Z h s   & 0 > J V c n z   ! - : E P \ h u )5ALYdp} $0<GT`ly !,8DP\iu'2?LXcp{ %0<IUamy"-;GTan{ ( %.+ *!'))2'>>>>>8!2#>>,>;+$&"";'"$;>  8;;;'""+.;8&>>>>3>>$&+         ""     "         "" %  $    "++3'&"--&8;;'2828>8-8")""'882>>8>8.882882 $ '2/'/8')288>8//8!'+  *4. ( %.+ *!'))2'>>>>>8!2#>>,>;+$&"";'"$;>  8;;;'""+.;8&>>>>3>>$&+         ""     "         "" %  $    "++3'&"--&8;;'2828>8-8")""'882>>8>8.882882 $ '2/'/8')288>8//8!'+  *4.>B5 :0 cHFOY8P"X7J:9)d'9,_I *&DLD'X6<-d? -B5BcI? -"OW)V!BB5!d.%3BaTB1aZ _*<B6FLP K+&O)\>BR6A$2$)IEXdJ2=:)%J;):TEJ#9BO'"U-K+7E[I5'!PH"7[\W G#:M4D$4,-D O ,P4T* :;??O`X,%'c0 ANQOH.3R>\I0`[+(&@I&.aTZ`>K<>*-C `'T.KT11$' c*+0E<,;B?4Y9!N.C0Y@!"U 5_b5 Z 4.>6L(@T I;>0[KOU2979S.4&. 3+XIA]E<J-:]>=1>QFK8.AZC( :2*4PE 94N[+9{CdSd77 lflx.mt?py  2ZrEZI/e*F\T;mj4[$Uw,jCt_Sa @.KzA6HJAaFartAkE=(Fw$3dOjpV|aU/XZd 9pGK{}+hLh:^La$L}.@@l<_\ 1${NT[;]]''.xAA]Idw2',Of%Gc ltQfbm L&,; z(s'jk& y ekzsQ h v]R,ut;}DL[-blUFHmWT@@/?/2E5C:XxK!% (,#(5+)*//'1V8wO87/ 3&$&_c]*66;#& ( <" +* <       N0287 $    @         ,6ip"F-/lM% Npt=+Vh};sJ Mp,M8l,FI:FB30h@zs-N,eMq.aaRF5p3.wx}2|o* `?P,F n&5'P%3@do1Qpvk6h:U:bD*)R&ddeh_A=U)`"xR]{q&R>8*Xb>Rzv,Xyd`I4%~~>jQ=rGd*("tVkGrV:.|(J[ SBp4P8B<"P3UP]E)!08/p@*rKB3Jm1QKGR#kk1OWL=8qllsdD)} oh)$2o)<UM`%@) S?FWFYjD7p*XDL*7h>3p{X&j68( @E6MU0UdY9g1 6@9P86.H~oB/5(&sE$BZGRA6L->8GT@]/K1/.L`Qo(U+ ,)Fur49eHbD7rE|jukTq!MHN?C&R[EkhR@yHPm3Mc/!t=9GG (GAGGCACCTTCTCGCGTTAACGACAAAATAATAACTTGGGTGATGTTGCTGAACGCGTAACCCGCACTTAGTGAACCGGTCTGTACATGCGGTGCGGTAGCGACGGGGGAGAATCTGCAAACGTGTAATAGTCAGCATCTAGAGCAGGTACAGGTCAACTTTTAAAATATGGATCATGCAGGTCAGTCGACTGAGCATCAGTCACACAATCTAACGTGACGAAGCAGCTCCTTTAGCTCTGCCCACTTTTCACTTTACTACAATCCGCCTGAATGGTTTCGCCTACAGCATTGACTGGGGGTCTCTACTCCCTGGAACAATGTCGCTAAGATCATCGCAACGGAAAATACCTCGGGGATCACGCCAGGAGAGAAGAGAAGAGTCTATGTTCTAGACTCTTTTTCCATCTGAAGGBCPFileName=KB.bcp;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=1;AverageSpacing=0;ProcessedDataProfile=1;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;KB 1.4.02010-08-05 17:19:39 +08:00AEPtZAEPtZAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'VVDATA 'V~#DATA 'VyDATA 'VDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FWO_GATCGTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANE=LASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOIS-P1AM<P1RL<PP1WD<P2AM<@P2BA|P2RL< PBASPBAS`PCONPCONPDMFVPDMFDPLOC<PLOC<PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%%SCANASMED SMLtSMPLVSPACA[SPAC=SPACA[SVER3.0SVER }SVER,,2Scal@ScanATUBEB7Tmpr<UserMBphARphCH[phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPtZAEPtZAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'VVDATA 'V~#DATA 'VyDATA 'VDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FTab"FVoc"FWO_GATCFeat//"GTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANE=LASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOIS-P1AM<P1RL<PP1WD<P2AM<@P2BA|P2RL< PBASPBAS`PCONPCONPDMFVPDMFDPLOC<PLOC<PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%%SCANASMED SMLtSMPLVSPACA[SPAC=SPACA[SVER3.0SVER }SVER,,2Scal@ScanATUBEB7Tmpr<UserMBphARphCH[phDYbigphQLcphTRphTRKj5)l#O,[h?Yo%"*"i/2Rd}/o66Q^_4e]"=v1r:zwpF*xvJ\S#!ZI@])HJV1pfnST/4f!Z56N|&ZG1&w%:T?1bTio0<57#u?GG5WriD$[Wtua 3nG6`V5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`>-?[G2upkyl,7hhSc>6?|$lQ%e+Bw9#MBz3)-l'CFbhLYr4EjuDs5iqbyMimE%ToN6|1:#<'r{?:TWsW,?iHF@LeP *">BZuGZ-p@vddD\4ApZTFS$oak"T'oA_AN;)oX6YMI){mnjH[F.A[s:>ZCCd+4k,Z)lO[vHds<-F3x[R2LxC{IF}oliAd9w"OMbA,}ktA!0p(WA!e~8~~+N@c'v+gK >o>s1HT+O}L6>U{mwY1XV7:>JW-z(Z=kT/n@6TdO{7>kOptpuze/WR/X2EtERPV]O8oe])%,8Ea-cZ,f*F8B,}sv&'Yj}4]'xP!t p7swnqR#$|5q_fU9~bUpZ*JUt._qfteuNy,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_t[p i,? W]H6X+9mvH%lFU?-4$&FkNp&iJ-[qr[&Xj|82Z~9}j~:HzAb9ECQ/}Z&['K2;+hj3ST?0sKG'BNp3JWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc[mC`#F*r`snm$7uM2^7f!]47.<-m2$YWmOyl}$rEGCN8m*1fCy]_^ave%e0MSayw}sbe/%ToN668d#zir8rP p'Ic62nFT16Y=*">InScInVtLANE=LASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOIS-P1AM<P1RL<PP1WD<P2AM<@P2BA|P2RL< PBASPBAS`PCONPCONPDMFVPDMFDPLOC<PLOC<PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%%SCANASMED SMLtSMPL VSPACA[SPAC=SPACA[SVER3.0SVER }SVER,,2Scal@ScanATUBEB7Tmpr<UserMBphARphCH[phDYbigphQLcphTRphTR