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                                                                                                                                                                                                                                                                            d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRE>IMMMKKIHFEC@?<;7520-*'$!  ^^^TTTxxx   $#*//9::EEEPPOZZYccclmlvvu~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258000-04 6258002-04 6258003-03 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f874a0eea05011df841900065bdd9e79seq900original KB.bcpKB_3730_POP7_BDTv3.mobtruefalsefalsefalse11.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000truefalse20false510false800false25.0falsefalsefalsefalse10true5502042020420falseKB.bcp# 5Prime Basecaller Paramters seq900original seq900original5LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5050Run_3730_2010-08-05_15-29_03790805001080500196-Well                                  704-DEFB4-RKB_3730_POP7_BDTv3.mob !"%)/48:<?BEHKNQUY^chlnprty~zsnid^XQKFA=:63/+($! 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K V c q {  - 9 G S _ k w    * 5 B N Y d q ~ '2>JUbmy "-9EQ]jv+6BNZfs %1=JWcmz $0;GT`mx$0;GTanz"5=Olx!)6AOW^iw )6COYcn{'1?LYgs} -9CN[gs&2>JValw+8CMYer$.:HT_jw*4AMYdo}  # 1 ; I U a m y    + 9 E R ] i v    ) 4 ? K V c q {  - 9 G S _ k w    * 5 B N Y d q ~ '2>JUbmy "-9EQ]jv+6BNZfs %1=JWcmz $0;GT`mx$0;GTanz% %)3) **$#)&&.*>>>>>8'&3&>;;>;+" 8'))1++>  1;>>,#(31;31&";>>>3>>+&.  """ ' '"'"" #  ""$""+"""" """ """+" ++"".."  (2""'"+02831+2&22"'"';;;+2;2>;8">")+&''+3'-+;88>>811&8888.. # +#$38>38>8 &.8>8/88*   /% %)3) **$#)&&.*>>>>>8'&3&>;;>;+" 8'))1++>  1;>>,#(31;31&";>>>3>>+&.  """ ' '"'"" #  ""$""+"""" """ """+" ++"".."  (2""'"+02831+2&22"'"';;;+2;2>;8">")+&''+3'-+;88>>811&8888.. # +#$38>38>8 &.8>8/88*   /2<:[)OZG?A!^VQZ U8V%b#@P0L,/H` [.8-J1GP-d*=IF7 C +B6BPE.J1 0FGH0`!87"J:@H:M4\ K*@4:T1_Q'U.>b9TH: :.HY; 62* =QAG''3OF8VH9WI`S< -FW<S= Z"?/S2,*QB3U X7N?W,G):",@;QM"ABFDN1 4JDa/49F.[V[;KI/#!] a+9R#@R%$a2G<-U9V3%WE2^A&;Y+JN.A8" &8%G>5GHTD )I091K% >$LO](A'3TZ^E7Q'21C *BT"[M%ZL"`^_R &S8]VV/F>9IB5L+B[D5=)A(NE)Y?N]%%-J7Y pAnOFeg8C_CJ6C_`OnOU#_]14vdD k]L-uk<'cE_un"|QKU]=M n:uat"F/pTl(BXiv\!P\K{@rho'O3Q62D._0)6;Xdzw~*!vg7SE{9d/o UW_S2W[,:v%NYw\A(5[4JN[)e 00n>n |t/ZTikt@,8 fB5g :E-{y",3 d98R Kw@yyxqzqbFldYV@DdKM@1<+->-:4xx8-#"( #+ &"!0*?8x? : "#3 32$,:- &'  5 3 $5 .    %  9&&" @%pgp{,"%$8d*pMdtVa2yp[q8ikL>[ pto9vXzGU\V.K4-gdRA5lyHp@H=$dhc?}=}z4~cleLYMxonS- *i`yl=#L!T8.l bld]Z:Snuk>>3?L\fx_~_ {F! x3qa.8TjP/@QFpp*T!%Q6L<U_uw_ixxl%Azff Ql]NEib3WK W*(<63#/S7Uf:Z:(qX%'$>QA}>D->tL^__+vU)KSm8_k?bUb>eXDb9bT{/us\CQ [$CaEdaP4i \,RX9'+&Q[_9mE&L+cM?Nq8.3eR_}=0f4=G$tycD7dt H'-!B@~`HC=-nEZOb(^L>HzVZ&*5+3<2%HQ|p@$jCgR9c-5AFLAubF`qlQnXN@1U4jG<|\ZTuTq?$tI.w}sOYF`CX,kvUFT> V@GGAAGCAAAACTCGCGTTAAGGACAAAATAATAACATGGGAGATGTTCCGGAACGACTAACCGGCACTGAGAGCAACGGTCTGCACATGCGGTGTGGTTGCGACGGGGGAGAATCTCCATATGTGTAATAGTCACCATCTCGAACAGGTACAGCTCGAGTGTTAAAATATGGATCTTGCAGGTCAGTCGACTGAGCATCAGTCACACTATCTAACGTGACGAGGCAACTCCTGTAGCTCTGCGCACTATTCACTGTCCTACAATCCGCCTGAATGGTATCGCCTACAGCATCGACTGGTGGTCTCTACTCCCAGGAATAGTTTCGCGAAGGTCATCACAGGGGAAAATACCTCGGGGATCACGCCAGGAGAGAAGAGAAGAGTCTATGTTCTAGATTCTTTTTCCATCTGAAGGBCPFileName=KB.bcp;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=1;AverageSpacing=0;ProcessedDataProfile=1;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;KB 1.4.02010-08-05 17:17:55 +08:00AEPtAEPtAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'pVDATA 'p~=DATA 'pDATA 'pDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FWO_GATCGTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANE LASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISP1AM<P1RL<P1WD<0P2AM<P2BAP2RL< lPBASfPBASPCONPCON|PDMFVPDMFPLOC<@PLOC<PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%SCANASMED SMLtSMPLVSPACA]8SPACSPACA]8SVER3.0SVER SVER,,2Scal@ScanATUBED1Tmpr<UserMBphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPtAEPtAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'pVDATA 'p~=DATA 'pDATA 'pDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FTab#IFVoc#8FWO_GATCFeat//# GTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANE LASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISP1AM<P1RL<P1WD<0P2AM<P2BAP2RL< lPBASfPBASPCONPCON|PDMFVPDMFPLOC<@PLOC<PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%SCANASMED SMLtSMPLVSPACA]8SPACSPACA]8SVER3.0SVER SVER,,2Scal@ScanATUBED1Tmpr<UserMBphARphCHphDYbigphQLcphTRphTR/o'zbMaQd&79?OkZL9,,vk1G&M6] 7UkAxBd?DSGwvlAc3_{#RX#W+BcK;o`H9T+a:WkR,uKsK[NHu` *R@_AiG3mwYgE@f9kf'C#/\+F\%_krjZUTw|bT8Poo=[/19~{yrA]#_WfOMt_X`@kjJ}B~xGH$i*8j|]]u.ibu@Qhiq|fq:#6D:_f7o,f9r;qBAK$M@at0<EkP9bA:>y7j] !3xnv_YDkrD5pYy7,Fb[2yy>T\3>>B]'p8Npd-(bcn23i4Fbrtr%+6z={n=zOE 3IG_3Th|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>V3jo>E7-8rj~n.It!TQ'O1^m?e({ln}sFZ;59])J3}+;&*6IlwY\j>pbT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D\^TE-\47.<2A2{UZBwE$X~}:>JqNRI1]f)yA@r9s_|g+VNwBx6Ks;6R&)Y?Z8Z~9K$^aC?^V$Yeaejp~{>ZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m*0_fs$X=<;#(eaK|`!t/[z~+7+>0"t5+'v\Rmqu2Ewuu~L79>(hZ$~I!|spN$t.Dd^4G^({C!RJ<6Q^Zew.k.d}uNf1&vM\#YTEl?4ubH6X12g[jWcgiQF4Np3`:/^?swA#Q9nKH{_?lv6qe%sSaycyShKn6g;X6YM%gcmeaAvG9 HtWo0<8WFoe~8Wbq S]*Cy KJFU'.Q1db{wMzR9v!w2l89+uTE#!#hmmfc4hACTT3T&^%FyPC,ComMOxq6agI){y&m"o`^ kS\KQpYp&!'\/.:AEPtAEPtAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtAUDTrr1\B1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'pVDATA 'p~=DATA 'pDATA 'pDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FTab#IFVoc#8FWO_GATCFeat//# GTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANE LASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISP1AM<P1RL<P1WD<0P2AM<P2BAP2RL< lPBASfPBASPCONPCON|PDMFVPDMFPLOC<@PLOC<PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%SCANASMED SMLtSMPL VSPACA]8SPACSPACA]8SVER3.0SVER SVER,,2Scal@ScanATUBED1Tmpr<UserMBphARphCHphDYbigphQLcphTRphTR