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(   '   #  "!"  d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRE>IMMMKKIHFEC@?<;7520-*'$!  ^^^TTTxxx   $#*//9::EEEPPOZZYccclmlvvu~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258000-04 6258002-04 6258003-03 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f874a0f1a05011df841900065bdd9e79seq900original KB.bcpKB_3730_POP7_BDTv3.mobtruefalsefalsefalse11.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000truefalse20false510false800false25.0falsefalsefalsefalse10true5502042020420falseKB.bcp# 5Prime Basecaller Paramters seq900original seq900original5LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5050Run_3730_2010-08-05_15-29_03790805001080500196-Well                                  708-DEFB4-RKB_3730_POP7_BDTv3.mob#'*-037:=?@ACFJNTX]`cfimrw|  |vohaZSLF@:4.)$    ")08@HPX_dhiifc^YSNJFCA?>==>?@BCDDDFIOXfzVSq&U0;umgb\ULD<4.)$  "+7CP[djmlie`[VSPMLLMOQRRQMHA:4/+(''(()+.4=JXgurcSD6-*/Bg{B Li"D o CoJ3))2@Rew|kXF6*"!$'()'$  +M}P dU/  1DUdpvwslcYQLLQYbjqsqlcXJ;, ,W O !n U<iTB2%   *5>CDA:/$ $0=ISZ]\VM@3'   +8EQY[XPC6+%#&,3;JgV<,>CU8.v 0}`TQNH?4+%$(,134:Iltkbo FIvdJl'$<Xvy\A* $1?MX]\SE2    +~  }zb"T<X#P  "),,'!&4DR[_]VLB930/1231+#  +=N[i}/M~MQ @'hK1   G';(H   .l3G_Ls%n]L;-"!%*06=CHJIE?94113685-!H;;9,_NF?8/'%)2?JQRMD9,!3]\ 64We?,(+1:FVjjL0Dcn}uM# I=HozBj#>~Iea@ cD3,)'&&(+/10) B.*OLB35xfx. &Cb}z]<  !=Yp}~s_E, +E`ur[A' !2@IJE:.##*.0/*$.[ 9>-}@"0:<6(,KikN1&>WktqcM5 "&$  &6IZgkfYI:/)'''$ !',15;EUjx`F, G#,0%6=26V@/C 2^u^o*+ESWVVXZWOC5)"!%+17;=>?@ADFHGEB@BGOX`efcYH/#P# o1<:aBSa* +38;;94,"    '.3651+$ /APXXOD:6:ER^fhe[L6 b8 jK~pVdzGxX</0=TpdF0',>Yux_G4%&Cq, NG$Q4288.   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'..$t ;* LBZxwV8>eJ1}p{ygWJ@80(!#+4;=90&#,33-%#0U9{aG/~1b=^wz[$'cF844542/+)'&&'*1=Qo }V 83b5" $))$  h @J#@ \Ai@ =KB.bcpKB_3730_POP7_BDTv3.mobtermTTGGTACCCCCTCTCTTGATTTCCACTGGATTAGCACCTTTGCTAACCTTCTGGAAGGCTTATTTGGAAATAGAGCATACCAGTACAGCAGCAGTGATAGTGCCATCCCCCAGTCTCTTCATCTGTGTTATTGGCAACATCTTGGACAAGTTTAGCTCCAATGCTTTTATATTTATCCTTTAAGTCAATTGACTTTGCATCAGTCACACCATCTTTTGTTACTTTGGGACTTCCCCAGCTATGTTCAATAATTACTGTTCTTCCCTTTGGCCCCATTGTAATGGCTACAGCCTCGACAAAAAGTCTACACTTTGAAGCATTAAGGCTCGGACATCAGCACCAAATTTTACATCTTTACCATCACTTCAAGTGAGGTGAGGAGCCAGTACCCTGGACACTGGTCCTCATCTGGATTGGTACCCCCTCTCTTGATTTCCACTGGATTAGCACCTTTGCTAACCTTCTGGAAGGCTTATTTGGAAATAGAGCATACCAGTACAGCAGCAGTGATAGTGCCATCCCCCAGTCTCTTCATCTGTGTTATTGGCAACATCTTGGACAAGTTTAGCTCCAATGCTTTTATATTTATCCTTTAAGTCAATTGACTTTGCATCAGTCACACCATCTTTTGTTACTTTGGGACTTCCCCAGCTATGTTCAATAATTACTGTTCTTCCCTTTGGCCCCATTGTAATGGCTACAGCCTCGACAAAAAGTCTACACTTTGAAGCATTAAGGCTCGGACATCAGCACCAAATTTTACATCTTTACCATCACTTCAAGTGAGGTGAGGAGCCAGTACCCTGGACACTGGTCCTCATCTGGA1;MXu#,9DQZ`kz+9ER\fq})4AN\jv #0<FQ^jv)5ANYeoz ".;GQ]hu'1>LWcnz "-7DQ\gs    ' 4 ? 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L X d p }  ! . < H U ` l y  , 8 C N Z f t ~  # / < J U a n z  , 8 E P \ g t )4@LWdp{ $/;GS_lw  ,7CP\ht'3>KXdo|%2=HVany"-;HTan{  '/) %%$!))&">8>>>8*'&3&>83;3+& '++"+.8"")+)';>   &.;;.&2"0;2 +";>8>1>>+&&   " '"'"" "  ""'  " ""( ) """#(" 1"  (0" ')32822'-"-+""3;.&2;8>;;";&""+3'--;888888&8882882#  %& %33".8*& $)8>8/88/88! *4*    '/) %%$!))&">8>>>8*'&3&>83;3+& '++"+.8"")+)';>   &.;;.&2"0;2 +";>8>1>>+&&   " '"'"" "  ""'  " ""( ) """#(" 1"  (0" ')32822'-"-+""3;.&2;8>;;";&""+3'--;888888&8882882#  %& %33".8*& $)8>8/88/88! *4*  Z:)Od82N_=5, G 8UUBWLZ1/*/ #7H.[_$&8@$ dQFQFO-SH,>@D3A40U.Q Vd%c''FA+NMTP@ >3'9_SLF]8+&CU-P-V#\&N("5VV*I(7bL;D<X R88X4Y2-H+"\8P;EWF2F<=/U"C #McJ+8\8L'4!$9R;Y -F?LVT>8PG1."!+@J8ADF#*`a' $9Z+MP= E^2!2%9cVN5%T 5O*BY3W bNB25O=>dD^(M,V+"3`3B4T-> ^DJU5 !GKP51:Jd] B%<O.V/F,-B( 969L %HTQXQ H\a=K^NB0A@'8,V@$*2@Z1+Kf`|=)0'SE S6! 5?>M%oIah/e-=O]'S/!Jc eZ[)X7;6,kQ KRIE2S$_p.#_Bj 0,/<&EI}gL.HLJVyA%@!fL2+;GrO1$~B-;/-w1Hiw y8j4L-aj[*\yG"aKWAjUiYG 7>%xK~*_ 's_4Ld_A&v7R{Y)iGgDj&Dq%  )|f\S9iJ{~NxlhgDQpYV7@35@1G7fx64,"(/%$.-+%!1/bUUK@.1)A(!MBT&AB71127("'N&E()-" LK          3  ! C+% '  $ ;,bXpB5p 8&5e>kF7p]?Q@po7v(:d@=sXZJZM+Zfx~;R=\8[pc6Q C8M>&V<{PwkefP_C pM%huiEksFl7,|90?L- ;X4B5Mxk7dF!+( dTd;"Z tGq7QOF%@?Ml;N])1UBbl^l>bVY3y\pdv0O}ar?]m|`v|pNZa-gh136WN4J8AC^Tb/]TG$0`aLvMV(M8NuSP\x0[1PYg4{}`C[tbMW,iCZPHUjJm)jo:F=E9]I#]?^;YUX>9TfFZ6eej]W<x{7nE0%YTVK%%_C#^b{?^kM ^Si7*L)<G0,Q !.M$,*Xxw;-ZA_ROB?mtVXZTFpHS+Tn[ak3hp>?xIgJf@P7!fo|.t8GGAAGGTAAAACTCGCGTTAACGACAAAATAATAACATGGGAGATGTTGCGGAAGGACGAACCGGCACTGAGAGCACCGGTCAGCACATGCGGTGTGGTTACGACGGGGGAGAATCTCCATATGTGTAATAGTCACCATCTCGAACAGGTACACCTCGACTGTTAAAATATGGATCTTGCAGGTCAGTCGACTGAGCATCAGTCACACAATCTAAGGTGACGAGGCAACTCCGGTAGCTCTGCGCACTATTCACTGTCCTACAATCCGCCTGAATGGTATCGCCTACAGCATCGACTGGGGGTCTCTACTCCCAGGAACACCTTCGCGAAGGTAATCACAAGGGAAAATACCTCGGGGATCACGCCAGGAGAGAAGAGAAGAGTCAATGTTCTATACTCTTGTTCTCCTAAAGBCPFileName=KB.bcp;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=1;AverageSpacing=0;ProcessedDataProfile=1;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;KB 1.4.02010-08-05 17:17:43 +08:00AEPtAEPtAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'hVDATA 'h~5DATA 'hDATA 'hDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FWO_GATCGTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANELASTccOLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISuP1AM:P1RL:P1WD:P2AM:xP2BAP2RL: >PBASEPBASPCONPCONVPDMFVPDMFPLOC:PLOC:PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%mSCANASMED SMLtSMPLVSPACA_ŋSPACSPACA_ŋSVER3.0SVER SVER,,2Scal@ScanATUBEG1Tmpr<UserMBphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPtAEPtAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'hVDATA 'h~5DATA 'hDATA 'hDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FTab#FVoc#FWO_GATCFeat//"GTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANELASTccOLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISuP1AM:P1RL:P1WD:P2AM:xP2BAP2RL: >PBASEPBASPCONPCONVPDMFVPDMFPLOC:PLOC:PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%mSCANASMED SMLtSMPLVSPACA_ŋSPACSPACA_ŋSVER3.0SVER SVER,,2Scal@ScanATUBEG1Tmpr<UserMBphARphCHphDYbigphQLcphTRphTR(w}Uzrl@O(LxC[>({]>REd7gbOHG'~[ABMNT@bxj[Dbt6XhVx9zG@[76gg`qC+lkfc40-fKbVU}MS{p[v[!s.#g;X6YM'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ-,Cz#Z|#e1a_y`[qD|3DG\TcpP.!3WN#4LdCh2d'jw~| imyZSH,6cXM1voDGG5)ljD_n?Kf]4'Uw\Pcc{\A_}$A>AR5Z(>B|::zWTR0Xwt2)lOY^k42g|5 x*.JIc\e:!9B_de"lopM".kQQT[dM:$M(P@>/D?*Uq$[-o9D4Dj&['K2;+hj3jT$3o%+0Oj!Q4]"{vz6$l>o XS_d.u?zD6~hlwXs9?Ou4FS7&3-OT5ro{`N%7`h?&0(B~W^17]GhnW"K-[llj_odF4NYHY8|!I,62Z5hf6]X?K90j2oFtwwj^A9}:>J5KRCmnlcEn=6;}S$T(-iw87=6"4#O.5k|_MgPV]O8oe])%|2CWllmeQQ6z%!SNmW.$7KO]eOY ~?p M;<`}l#[GVsI.J:*yEwiOfF{HB*@Yy-4$&+VN;172e,qz7|!Y<,h`Fzmj7Ul*Qml>R7RKng_[g!CCq "I,:MGk;0|5Q1V?|&@8ja:3AGXS<< xrTW?iMAkc PkIp?H^Poa#]}Ko)6V8JX|K2-!;9xxsWD@|DmOY,f *" `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF}vTJ}e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~bea6:!.tk}Y=52rR~o[k7MLr92U*a!>InScInVtLANELASTccOLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISuP1AM:P1RL:P1WD:P2AM:xP2BAP2RL: >PBASEPBASPCONPCONVPDMFVPDMFPLOC:PLOC:PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%mSCANASMED SMLtSMPL VSPACA_ŋSPACSPACA_ŋSVER3.0SVER SVER,,2Scal@ScanATUBEG1Tmpr<UserMBphARphCHphDYbigphQLcphTRphTR