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                                                                                                                                                           #                                                                                 d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRE>IMMMKKIHFEC@?<;7520-*'$!  ^^^TTTxxx   $#*//9::EEEPPOZZYccclmlvvu~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258000-04 6258002-04 6258003-03 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f874a0f2a05011df841900065bdd9e79seq900original KB.bcpKB_3730_POP7_BDTv3.mobtruefalsefalsefalse11.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000truefalse20false510false800false25.0falsefalsefalsefalse10true5502042020420falseKB.bcp# 5Prime Basecaller Paramters seq900original seq900original5LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5050Run_3730_2010-08-05_15-29_03790805001080500196-Well                                  709-DEFB4-RKB_3730_POP7_BDTv3.mob9:<@FMSVXXX[_djpvz}|xrkc[SMHDA=93.*&"  ")07>EIMOPQQQOMHB:3,&" %+19ALZk!_A#]}M c cxfZRLFA<72-($   (1:DMT[_``]YTPKHGFHJLOPPOLHC>940-+*)))))**+-.035789:::8630..4B^DU5r(r.\CW0}pVmA'*7FUdpy}{reTC4& 'J|X40?d5   "0?MZbfe^SF7*)3>GMOKC7*#E<c] X0_F7-&! 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"#$%'+18=>;3(!%'%"<K;}5Lq> MHyyIaZ->YZ%<V5UP4QejaP>0*,5@KSURH;,     7fnR` LT|4IxG@Y ZKjC+F+RF    %5?B=1" 0KcrthQ6  E 25gJGZx|Y4 !<Ugoj[D*?tro+sdNBQgslU4 WFv{QO.\:  $O id3A=!TXHiAS^(-fYuc,{u!>j<\L +M{%^C|w3>`"     (HljB-D*v =sg?Hm{[@0,3@LNC*pF!\V+1PqxY1'nh~A$*0xO\C T=@YzxT3/BQXWM>- ",, *[ NU,9:0  -@PXXN>-*<N\b_UE5&  #-;NbthF" >0v lq7);\}a:!.tXNK<I^kiZD-  &19:1$"IGVB#05/$ #8IPH5 =<n$lp@8EWac]VQOOMJFCCEFA6&$MI-r\bt~hE&D7Q=3I[bJO"2;9- ->JQRK@2$     0COSMFLqELr3pK,-E`vmK)7.1MTzl2*>Xhi]H1  /AJF3 NTD:x@HU( (.4:CNZentvqcM1.u{h`,96'%cOQOLZAvh88Ra^H%" s1eG *+!         #.:EMQMC3" +>LOD. au&:^*eBt@Sxd40AWeg_QB5*"  *3884+! $XG}uB zAWK "46XaGTowaJ6&  (.* QV YLaFWxjA,Nylh5%\sf`ipmbRB4*     +[<]X:gI) ukdbfr9xFlUoH1'"  !5WAI8w}FJW( R  ?@w@@>B@KB.bcpKB_3730_POP7_BDTv3.mobtermAGGAACCGCTCTCTTGATTTCCACTGGATTAGCACCTTTGCTAACCTTCTGGAAGGCTTATTTGGAAATAGAGCATACCAGTACAGCAGCAGTGATAGTGCCATCCCCCAGTCTCTTCATCTGTGTTATTGGCAACATCTTGGACAAGTTTAGCTCCAATGCTTTTATATTTATCCTTTAAGTCAATTGACTTTGCATCAGTCACACCATCTTTTGTTACTTTGGGACTTCCCCAGCTATGTTCAATAATTACTGTTCTTCCCTTTGGCCCCATTGTAATGGCTACAGCCTCGACAAAAAGTCTACACTTTGAAGCATTAAGGCTCGGACATCAGCACCAAATTTTACATCTTTACCATCACTTCAAGTGAGGTGAGGAGCCAGTACCCTGGACACTGGTCTTCATCTGGAAAGGAACCGCTCTCTTGATTTCCACTGGATTAGCACCTTTGCTAACCTTCTGGAAGGCTTATTTGGAAATAGAGCATACCAGTACAGCAGCAGTGATAGTGCCATCCCCCAGTCTCTTCATCTGTGTTATTGGCAACATCTTGGACAAGTTTAGCTCCAATGCTTTTATATTTATCCTTTAAGTCAATTGACTTTGCATCAGTCACACCATCTTTTGTTACTTTGGGACTTCCCCAGCTATGTTCAATAATTACTGTTCTTCCCTTTGGCCCCATTGTAATGGCTACAGCCTCGACAAAAAGTCTACACTTTGAAGCATTAAGGCTCGGACATCAGCACCAAATTTTACATCTTTACCATCACTTCAAGTGAGGTGAGGAGCCAGTACCCTGGACACTGGTCTTCATCTGGAA,=Pf %2=KSZet (5BNXcnz(2@MZiu #0<GQ^jw !+7CO[fq| %0>IS_kx  +5AO[fq~&1<HUakw    + 9 C Q ] i u    ' 3 A N Z f r     & 2 = H S _ l y    ( 5 A O [ g t     & 2 = J V a l y  ".9EQ]iu(4@LWdp| $0<GT`lx*6AN[gr(4?KXdp| '0<IUbo|,=Pf %2=KSZet (5BNXcnz(2@MZiu #0<GQ^jw !+7CO[fq| %0>IS_kx  +5AO[fq~&1<HUakw    + 9 C Q ] i u    ' 3 A N Z f r     & 2 = H S _ l y    ( 5 A O [ g t     & 2 = J V a l y  ".9EQ]iu(4@LWdp| $0<GT`lx*6AN[gr(4?KXdp| '0<IUbo|      *" ))&&4*>>>>>>*!&2&11&1;+"'')"8"")1++>>2;>>,"3".>;"0#"+;>>;1>>1..  """ " '"'" #  """"+""  """( "" "+&+-"  '-"""")228322 )&838&888>882>&!'3&&888888>>888/.88"#" !+4>*44. #'4:)$*/2.4 "4'#)      *" ))&&4*>>>>>>*!&2&11&1;+"'')"8"")1++>>2;>>,"3".>;"0#"+;>>;1>>1..  """ " '"'" #  """"+""  """( "" "+&+-"  '-"""")228322 )&838&888>882>&!'3&&888888>>888/.88"#" !+4>*44. #'4:)$*/2.4 "4'#):d94T";O>N]F9H#@/M6a06:IHE=<_-B;"-YL 6["FKXUC2a C%*K 0HH<KNN*S&I"O ]>9/@/!7::YT9:a8 O> bAP=YA*D'F$DM> %LG(G/?*U&/?.KDDV(O-.F >#,K*]4 PAC BZ(GQ7RAb4P6Q#8!*,F\K .<(X0Q'PXAEF =JM6H%F8bXA;4'/`C;^@,& ;;5HcVC I 1B4UA1V;*DY:N5*_VC@YJQL]'Ia*[^M"B"+X5HT*4=N8;aV[=BCNT5Q\ )0N(X?JGcE*JD>Z" ,U7<H+!?.H46!S #`DOcG#W 3BB86Q_#(q@/T  "SAWIv['FQR.slSmvW4#+`$)Hy.=:-6B"44\zc -Neu/4gp^TK-WXu"WD|aFF$,L3aGhE41Jwc1 V"(,H+[7GNX Fu+qRp:(U#jg5\f(QY*!~[]|o%8#)= =H,P*o=Bttpb'e4s@zGx'UZ, FOCj P H T d1V2|'Zt"'dwlF9`D7af_DMjY[:D67C1E7lx@5,'+-)':50)'9)66 G(925#@5F66(:"$ #( (@#)*:-+&<;                         4 %  ' J;07) zdS!9: <O9Pcrfp, pS/A~z[GCIC1SP3m,pwYhG Q;OCJO,w;|;&$?rm/[u n^Pi9~ l DGp~p;Cyc73,;px!QP@#&drGGkzGAr^9!dc < Q)NCH:p}*K[~+w "EF8?7e8hd*jvK6o{'8IdE%ZrizPv+t7ER'bMMO4HG9FUTDJ=a\c)PAB./:<')@m,:Ga)"kR#JJ:v+3$QT\3EP>>L]s2(A^.-`7wS@J8%;NO% -vm=|OO5V-u9J{Y?9e*.A`h11!C5RWN=7h67i'_qx/!O^F2,tr@VPw52*%DMN9 !.flJXUx;bXC ZXb:1W''@ yu)>,,mvGlhOf-DCNKTaF3cKBPrL2l:?>{_IatN]frr%NBo th>WBa5L},smal7|^|eew.GAAGCTACACTCGAGTTAAGGACAAAATAATAACATGGGAGATTTTGCGGAACGACGAACCCGCACTGAGACCACCGGTCTATGCATCCGGTATGGTTCCGACCGGGGAGAATCTCCATATGTGTAATAGCCACCATCTAGAACAGGTACAGCTCAATTGTTAAAATATCGATCATGCAGGTCAGTCGACTGAGCATCAGTCACACTATCTAACGTGACGAGGCAACTCCGGTAGCTCTGCGCACTATTCACTGTCCTACAATCCGCCTGAATGGTATCGCCTACAGCATCGACTGGGTGTCTCTACTCCCAGGAAGGCCTTCGCGAAGGTAATCACAGGGGCAAATACCTCGGGGATCACGCCAGGTGAGAAGAGAAGAGTCTATGTTCTAGAGTCTTGTCCTCATAAAGGBCPFileName=KB.bcp;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=1;AverageSpacing=0;ProcessedDataProfile=1;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;KB 1.4.02010-08-05 17:17:38 +08:00AEPt AEPt APFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'bVDATA 'b~/DATA 'bDATA 'bDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FWO_GATCGTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANELASTcc$LIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOIS]P1AM8P1RL8pP1WD8P2AM8PP2BAP2RL8 PBAS,PBASPCONPCON8PDMFVPDMFtPLOC8PLOC8PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%USCANASMED SMLtSMPLVSPACAb-SPACmSPACAb-SVER3.0SVER SVER,,2Scal@ScanATUBEH1Tmpr<UserMBphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt AEPt APFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'bVDATA 'b~/DATA 'bDATA 'bDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FTab"FVoc"FWO_GATCFeat//"GTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANELASTcc$LIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOIS]P1AM8P1RL8pP1WD8P2AM8PP2BAP2RL8 PBAS,PBASPCONPCON8PDMFVPDMFtPLOC8PLOC8PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%USCANASMED SMLtSMPLVSPACAb-SPACmSPACAb-SVER3.0SVER SVER,,2Scal@ScanATUBEH1Tmpr<UserMBphARphCHphDYbigphQLcphTRphTR,qJ'8f7}7a2&d:Dp$^HA.tHZKo][571\hNQ0J;9 |!$!o%QG{c!%88 =![z)+^[,MZ@Y+P'#:&iChk}`:r&^?HYTw|bT|MI>"J268[`#|D5n4]p%!5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B``,#Xr_<.^ioA+7x,"L-2QvA-zI?I,z|>[mLoCDaD/|8i.bb>~ n3]NE^O{Nf7{hAh.iOWhbk1^wK[*oCf!`LY&'!"|C+CCW2T6D3t:44M1#H1%M8T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? s>}J8kOAWm ;op$SqwgE6FM;v&KT_ZO~|Q,r.~.bf0/Gc:(TZ*k|t7^Bwfmk=;A{%iTK(>V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3*-CdM]4e):EGa9?QLwUF3TP.&"D|;f[5kr+E{|`pQcWV=rDl)sp]jzUZ+1(F}z2g[jWcg;Z6Obe1Cd}Fp82)XaM~6>udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOA{%i/"@>oW~!.eHB,}&s-@M+9mW'>YP~jM.9Wl[Z,9AEPt AEPt APFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtAUDTrr0B1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'bVDATA 'b~/DATA 'bDATA 'bDCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FTab"FVoc"FWO_GATCFeat//"GTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANELASTcc$LIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOIS]P1AM8P1RL8pP1WD8P2AM8PP2BAP2RL8 PBAS,PBASPCONPCON8PDMFVPDMFtPLOC8PLOC8PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%USCANASMED SMLtSMPL VSPACAb-SPACmSPACAb-SVER3.0SVER SVER,,2Scal@ScanATUBEH1Tmpr<UserMBphARphCHphDYbigphQLcphTRphTR