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 ^^^TTTxxx   $#*//9::EEEPPOZZYccclmlvvu~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258000-04 6258002-04 6258003-03 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f874a0f3a05011df841900065bdd9e79seq900original KB.bcpKB_3730_POP7_BDTv3.mobtruefalsefalsefalse11.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000truefalse20false510false800false25.0falsefalsefalsefalse10true5502042020420falseKB.bcp# 5Prime Basecaller Paramters seq900original seq900original5LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5050Run_3730_2010-08-05_15-29_03790805001080500196-Well                                  710-DEFB4-RKB_3730_POP7_BDTv3.mob47<DO]jt{(<Ri|,CXk'Gc|y^@kP2gL2xnf`[VQKE@<:98630-,,.010,'"  !4I_u taPC:425=GTcr$Jw/B!Jc-5o&dL4 xqliggfffgimt"066.  wdSE:3-*(&$###&)/6>ELPSVY]bhou{[ t < U  R  f XcEj]\X02&Gbv~{kO,mb\XVTROLIFDCBB@?>>CPj {4|f_xK, #&()*)'# +GesU9%)=TkydSRp5 =N1bI8.*+-/0/-+**,.00/,'!&2BTdotrhXF3"  *=PcssaL5  $).49<<::B_=t6\V8+"xAdTQSVY\agmqqlcZV_}qTSpk+Nq{9    6WzxT4   "#%(-362%6mz3@o}x u2^/'1:AFKORROIB91,+0;Laus^I7*! 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(javVR s%Q1    +BYinfS;%&@b}_D45Hfsogz=wzHd(k0[Yp?uGdm@ " %%" ,@TdmmbO7  '' ODKJAS     $cmA;  97  x!$%  !MCJ.uF.'*-+"  %5AFD:+ "2z&Z\-H7Ywh  &7CIF;+     P)< b^u    %5ERXXQE6$&`1C(U m, .nd}`{@cld_  %(&""" 5{-m]v% Nt:;Ss[HMOE|>c7n8s0|s"P.  `&|G[fT     "09;4(    H'D0pxi$*'Z>  $7HRRI9& #&$     Ks2T H2,367:;7+2nC-T?I[|Twhx}p]GCTat6$*5:71*% #G:=5#oBEWA T?@N[_VD. &[+vM:;ENPI<- )q2xm,Iez+(  #!     .s(kl,O44DWa]K/]"t7U(sZ    "$# 5X"R/4d|; P ij}gr-kJ nHvJ0;d~u[@)"0?LROE7'$&%!  &W\b In$|#QpSL47[IXth<rI     '6AC>3$  2FTWN<'7u-2Q.'7Rjuq_E**>P[^WH2#X*}EGASv3':Wkn_D&B6q~TOJVA+:Zu; ,\(Q<g5$Q1R{n_V +m2{|4v@LLrx+aD  ;q.B-Y6O4[Q,>       3SvY$W,B$:W@#<o~[@0-7I[bW9 Zt%QH(8`^,Rg Vq)z)]iNL/.EiZ4 3NdpnaL4    $ gb9I; $24+ #! 0AMQMB2!2H^q}}nQ+a5 p]2Qgm`B/aA9eO0N__P:&  ,:@9&Ub ^= *7:4) !0<?6 YcmWO*APURMJKOQROKGEB</ *v5"YE%-IfvnS1,tn({x][BA&24.#  %6ENPH:(   &-+!&gB*'uckyxeL5$'7GOI4AlY&b5HMD5&    (6?>0*q>& P[\M!|Ia*".59<BJT`ktyyp]A$#^];/ 3<6#[Mdx$I N)!6N`cR1 ab;e?%592&   )6?B@8.#  %172" BIA~_6|`CmsS/K]aXG4# &/463-& #""S1`kNQDv3'FE0=61QiqiYH:1-*('%%%%$"#&((&!+:FE4/R(3mc09`tG)D\j`v|erweXROKE<2,*,28<=:62149AHMONMOYn8u{U/ L2cc DrUHDEEC>81,))+07APf3au zAm<('-21*  m Wz @@6@@KB.bcpKB_3730_POP7_BDTv3.mobtermTAACGAACCCCCCACCTCATGATTTCCACTGGATTAGCACCTTTGCTAACCTTCTGGAAGCGCTTATTTGGAAATAGAGCATACCAGTACAGCAGCAGTGATAGTGCCATCCCCCAGATCTCTTCATCTGTGTTATTGGCAACATCTTGGACAAGTTTAGCTCCAATGCTTTTATATTTATCCTTTAAGTCAATTGACTTTGCATCAGTCACACCATCTTTTGTTACTTTGGGACTTCCCCAGCTATGTTCAATAATTACTGTTCTTCCCTTTGGCCCCATTGTAATGGCTACAGCCTCGACAAAAAGTCTACACTTTGAAGCATTAAGGCTCGGACATCAGCACCAAATTTTACATCTTTACCATCACTTCAAGTGAGGTGAGGAGCCAGTACCCTGGACACTGGTCCTCATCTGGACATAACGAACCCCCCACCTCATGATTTCCACTGGATTAGCACCTTTGCTAACCTTCTGGAAGCGCTTATTTGGAAATAGAGCATACCAGTACAGCAGCAGTGATAGTGCCATCCCCCAGATCTCTTCATCTGTGTTATTGGCAACATCTTGGACAAGTTTAGCTCCAATGCTTTTATATTTATCCTTTAAGTCAATTGACTTTGCATCAGTCACACCATCTTTTGTTACTTTGGGACTTCCCCAGCTATGTTCAATAATTACTGTTCTTCCCTTTGGCCCCATTGTAATGGCTACAGCCTCGACAAAAAGTCTACACTTTGAAGCATTAAGGCTCGGACATCAGCACCAAATTTTACATCTTTACCATCACTTCAAGTGAGGTGAGGAGCCAGTACCCTGGACACTGGTCCTCATCTGGACA*8?LSr{ &/:FT\cn| /<IU_it #.9FS`o{(6BLWdp|&0<HT`lw*6COYdp}%0:FT`lv  +6ANZfq|   $ 1 > I V c n z    , 8 F S _ k w    + 7 B M X d q ~  " - : G T ` l y    + 7 C O [ f q ~ '3>JVbnz "-9EQ\iu)5AMYeq} $/;GS`mw !-9EP^iu(5ANZgt*8?LSr{ &/:FT\cn| /<IU_it #.9FS`o{(6BLWdp|&0<HT`lw*6COYdp}%0:FT`lv  +6ANZfq|   $ 1 > I V c n z    , 8 F S _ k w    + 7 B M X d q ~  " - : G T ` l y    + 7 C O [ f q ~ '3>JVbnz "-9EQ\iu)5AMYeq} $/;GS`mw !-9EP^iu(5ANZgt  #   #'#!404>>4!&/&>8'.$8 ,'$%;'")+)'8>   1;>+'(+3;02"";>>>;>>> $'   "+"""   ""+"" """" ""'" """%%""+1" " "0"")$"13820&2.83"""8>>2;;;>;;2>"'-''+.&"2-1;;>>;>>;>;;;;;++'""  %#'2,8>'383 **8>8%8888>  333'&8>   #   #'#!404>>4!&/&>8'.$8 ,'$%;'")+)'8>   1;>+'(+3;02"";>>>;>>> $'   "+"""   ""+"" """" ""'" """%%""+1" " "0"")$"13820&2.83"""8>>2;;;>;;2>"'-''+.&"2-1;;>>;>>;>;;;;;++'""  %#'2,8>'383 **8>8%8888>  333'&8> #HU6+1AK1F3_aX4;;.bDN9.P$R6G 7 1_+>[N(&5a-VWV.T, KB+ "$5 70W!2=)3D=]E$G7D+=L=1[R.)9Xb0/E69aEI3NA,< !SABIJ X6'D^BM$<69"ASbDa3:`#2A2HL *\=S.@2^@0 Z:57(K ,X`=JM CR;CH %'HY>##2E+$6W_[5<D5A&!G2W[M<>/b: P<E3+F<2OVMR 2G5&J7\?0L ?U@"b[H<VEMQ ^'L\*^cO&)C*/c!7U"_A$$I(JdEN SDRc'" -O'*AG# Ba2\IYN)Z) 1GXIDYA8F-@ K 8S,X0&&!;AX<<()\_(*XQ(3.7]P*l?r;* /mup?* Htz`ec7XDR2Y! #l~EVEsF_YD3W0PE Pe]zY _e%t~+:O.HnUqh%M~/RgZo`F-(FZ? m M r'*sX3SJ.2FC5hP[%zC ?pRw!v3? X]\Jr,b LqaAf* $e\y0 E_LQ3Z:1v< S#lK eW|p1j[H7je;klX]md?u [g9 G\ aIG[edZT&M}luSkrjEWyd_@5@14Q6E<SxB5,(//#-.**,#'1B5qM3344$+$!" Q)6,/   ((&6      !" $!l1#69h{ 0$:\@e,8'fFC\$W~j8Z=iKM|gM4K~jn T7a`eT#RQt?3}46s-dheOL;=8bT,TB.ba<xVAMp0M63=GKQZ?cKrSFS\nnFB}+!,Pfo" puY2x"Q&[x oV=iA _cMO-5H XdtSVd V_T<4Zy 8%S@E0sWhbIMz\1ssf+<f{DXWOJ0WHxvmQ$z3!]XaoGW4maCQL%qYKO.FnY?+(\:Y8PSpTX-n ^J:]T-8 5<Xu'.4-LAf/` c@}kbK.32mM[K~qDKBJI+pveI*s8:2UDZOG3  wGnm<k7K  rhkDJA,TK>'N/bF=,{_y? x*W1<JSxnrmt!vOM_ISsSDg/nS(NtnsSGy;oJ^x1XQGUrnKgAP:<,bU{]) !~CGGGGAGGTTAAAACATGGTGTTAAGGACAAAATAATAACATCGGTGATGTTGCTCAAGGCGCTAACCCGCACTGAGTGCACCGGTCTGTACATGCTGTGCGGTTGCAACGGGGGACAGATCTCCATATGTGTAATAGTCAGCATCTCGAACAGGTGCAGCTCGACTGTTAAAATATGGATCATGCAGGTCAGTCGACTGAGCATCAGTCACACAATCTAAGGTGACGAGGAAACTCCTTTAGCTCTGCGCACTAGTCACTGTACTACAATCCGCCTGAATGGTATCGCCTACAGCATCGACTGGTGGACTCTACTCCCAGGAATAGCTTCGCGAAGGTAATCACAAGGGAAAATACATCGGGGATCACGCCAGGAGAGAAGAGAAGAGTCAAGGTTGAATTGGCTTGTTCATCTAAAGAGBCPFileName=KB.bcp;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=1;AverageSpacing=0;ProcessedDataProfile=1;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;KB 1.4.02010-08-05 17:18:09 +08:00AEPtAEPtAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'VDATA '~gDATA 'DATA '͛DCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FWO_GATCGTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANELASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOIS=P1AMHP1RLHP1WDH P2AMHP2BAP2RLH hPBASPBASpPCONPCONHPDMFVPDMFTPLOCHPLOCHPSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%5SCANASMED SMLtSMPLVSPACA^SPACMSPACA^SVER3.0SVER SVER,,2Scal@ScanATUBEA2Tmpr<UserMBphARphCHkphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPtAEPtAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'VDATA '~gDATA 'DATA '͛DCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FTab$cFVoc$RFWO_GATCFeat//$#GTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANELASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOIS=P1AMHP1RLHP1WDH P2AMHP2BAP2RLH hPBASPBASpPCONPCONHPDMFVPDMFTPLOCHPLOCHPSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%5SCANASMED SMLtSMPLVSPACA^SPACMSPACA^SVER3.0SVER SVER,,2Scal@ScanATUBEA2Tmpr<UserMBphARphCHkphDYbigphQLcphTRphTR'%N`!2FPu4WyYZ(Wa:nuXK|+U17ujZj_!p-">R2`Yyb oJH.`Ep2=TgVSQ<}LgU!IZHp#)+KT[^[G^%E:"*l#qN8>o Ueh,9n5NJw=;SocJyw*6TdO{7I{4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/ud?7OBmxCB#v6|k.j H_H-CI;{dY7cI*]5cP}Gs+OStxHlYxC?(cU/~!-,'bF4$T9tR3#=cC0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4ZI,|Q|T{\K71P3;fTR[fRr;-6U@;EGCfQ&HQ$ 0603XXbLmX;9 |.W162Q$OEHO6z7B-_8Es{sMV^@}Ouxg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2s JX0bt-] 5y$47y 4orKOR`{A,}@1%i`$|BOsnT*NEU&mQD(bc p7DhcL(+gbk1^DR!_TcM#yKA[,5SpBoz?]HqQIxw}H{-*<@ZWeoa-iw87=6"4#OH}7a2&dAU@&k=.h^n@EK5llmxX|L=6X12g-4L7=x/D?*Uq$[-o9D4k,E-?){mnjH[q?Eb,8#V>AxYX]Ln?,}&8)TK7<4Zm[E,f *"c{+cWL N*hbDk0'_Cm ixPPis|K/l3672'[)VBZ$z.NyEwiOhiksyP:r~64L&eG1_bRohj4TIx*&`f5U_9} wvr]LaiY2]kO~JcW`$ `[2gx+C!K}Z'BNp{ e,&o.jax3o^b,:V AmE$ww*{we*V&1h.iOWhbk1^wKB_,qz74*le_M%~'pEA({]/AT^ogqlp,4lpy]AEPtAEPtAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtAUDTrr2vB1PtB1PtBCTSBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'VDATA '~gDATA 'DATA '͛DCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FTab$cFVoc$RFWO_GATCFeat//$#GTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANELASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOIS=P1AMHP1RLHP1WDH P2AMHP2BAP2RLH hPBASPBASpPCONPCONHPDMFVPDMFTPLOCHPLOCHPSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%5SCANASMED SMLtSMPL VSPACA^SPACMSPACA^SVER3.0SVER SVER,,2Scal@ScanATUBEA2Tmpr<UserMBphARphCHkphDYbigphQLcphTRphTR