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 ^^^TTTxxx   $#*//9::EEEPPOZZYccclmlvvu~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258000-04 6258002-04 6258003-03 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f874a112a05011df841900065bdd9e79seq900original KB.bcpKB_3730_POP7_BDTv3.mobtruefalsefalsefalse11.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000truefalse20false510false800false25.0falsefalsefalsefalse10true5502042020420falseKB.bcp# 5Prime Basecaller Paramters seq900original seq900original5LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5050Run_3730_2010-08-05_15-29_03790805001080500196-Well                                  743-DEFB4-RKB_3730_POP7_BDTv3.mob#5Sx pX@)~mZE.vi`ZUOH?4+#   !'.7BO_p{pha^\^afmt}8d4Vw % |'R\?)scTG<2+%(5FYoukbZTPMMNQUZ`fnw rzD {ckAM21YzVC?G[w*(oS=-#!"!$5Q{/ly<yI& $8Rr|aNFHSf|sYGF_H& w>p/eH2$ -@SdqxxpcQ=* #7Nf}s[D/   "%(+.1369;>ADGJMNNMO[|3BITFO<A/u [/xK4+)*,/259=??<99C\0UfJDo`W"*Ip!7;- \=)"*3;BFGGEC?;60+((*+&XQ~RHo*&L*s* !+4;>>;61./8G\rjUB4,((*-1442.**0@VqaIi]$ZF  D+.80D!'*+'  0}~;@~-p/lYE2#   '/6:<92'&Lw{'6pZL@2%"2EXfkh^P@2((5SqSj=!)Di[:&%;i|G>rj=$"%"8BmRB .m0LK,~L)(7GWdjh[F, J  v\#K' %`wpnt(&DfxT0,Kg|qY=# 6Us|bD) );IQQI<-   (P~ MOz1 (372&'Ff}qT5 .H_nqgT;#    +C\r}p^L=2+'%# !&-7DTfuyjXB)Z4G$kM5`$=Bp@Q#),2;BD?4'#+28<<:642110.+'$!"&-7>?4 LV\}M2a8&o4c?0h'-00-&    &4DQYYRE5&  .>JOKA3%"/<GKE2Y.}:DH&h9-5?vq@"+Ec~lO4!"4LctzufR=,!(>ce?nZ S,!"$  3ay'a<  #.8@FIHD=4+$"%'('&&'*,./.-,+-16;?DHID4mu,=`C*E!u"  /?JOOKD=4+! 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"3BG@- Xh5^xRv6+F%",7AN^prY9*Yh4O!)?ML:,,P$3QhC11@Vjvs_<:nO1aX1DIB3"  "$ '02.%  )5:7.   (4AMX^_XK:&  "9Sm~mIBm;s/$'t #D^f^L7'   +5=BDB>7.% AA<H,pt#84,T` 0q<DktQ4  "$$!(491k_p>Ho[#3+';zveC()3:?BCB?:41/1467534:H`:Wp}vk\K6 Aa6MZV< y%hL>98740,)((*-3=MeSP!%0*o=%,@K^@MA@KB.bcpKB_3730_POP7_BDTv3.mobtermGGGATCAACCTCTCTTGATTTCCACTGGATTAGCACCTTTGCTAACCTTCTGGAAGCCTTATTTGGAAATAGAGCATACCAGTACAGCAGCAGTGATAGTGCCATCCCCCAATCTCTTCATCTGTGTTATTGGCAACATCTTGGACAAGTTTAGCTCCAATGCTTTTATATTTATCCTTTAAGTCAATTGACTTTGCATCAGTCACACCATCTTTTGTTACTTTGGGACTTCCCCAGCTATGTTCAATAATTACTGTTCTTCCCTTTGGCCCCATTGTAATGGCTACAGCCTCGACAAAAAGTCTACACTTTGAAGCATTAAGGCTCGGACATCAGCACCAAATTTTACATCTTTACCATCACTTCAAGTGAGGTGAGGAGCCAGTACCCTGGACACTGGTCTTCATCTGGAAGGGGGATCAACCTCTCTTGATTTCCACTGGATTAGCACCTTTGCTAACCTTCTGGAAGCCTTATTTGGAAATAGAGCATACCAGTACAGCAGCAGTGATAGTGCCATCCCCCAATCTCTTCATCTGTGTTATTGGCAACATCTTGGACAAGTTTAGCTCCAATGCTTTTATATTTATCCTTTAAGTCAATTGACTTTGCATCAGTCACACCATCTTTTGTTACTTTGGGACTTCCCCAGCTATGTTCAATAATTACTGTTCTTCCCTTTGGCCCCATTGTAATGGCTACAGCCTCGACAAAAAGTCTACACTTTGAAGCATTAAGGCTCGGACATCAGCACCAAATTTTACATCTTTACCATCACTTCAAGTGAGGTGAGGAGCCAGTACCCTGGACACTGGTCTTCATCTGGAAGG!2NZoy'3BIP\jx,9FOYeq~*8ER`lw (4>IVbo{ #/;GS_it)6BLXcp~#.:HT_jw *5ANZep~  $ 2 = J W b n {   , : G S _ k x    + 6 A L X e r }  " . ; H T ` m x    + 7 D O Z f s  (3?KVcnz "-9EQ]ju*5AMYer~ $0;HTakx !-8CQ]it+5BN\hu!2NZoy'3BIP\jx,9FOYeq~*8ER`lw (4>IVbo{ #/;GS_it)6BLXcp~#.:HT_jw *5ANZep~  $ 2 = J W b n {   , : G S _ k x    + 6 A L X e r }  " . ; H T ` m x    + 7 D O Z f s  (3?KVcnz "-9EQ]ju*5AMYer~ $0;HTakx !-8CQ]it+5BN\hu    $   ) )+8.!!3"8&8 "$$8')+''3;   .;;1(+333"2&#2>8>3;>>3#" """" ) ""' #"  ""&""" "" "( "  """#%"&&" "2""""'"+2 83.'.%1'"'&%2;;'2832;8';-33+".')+3'--883;;81&-82--22++"""), &%&2#''38#888,3332>3%38338'  * 8.    $   ) )+8.!!3"8&8 "$$8')+''3;   .;;1(+333"2&#2>8>3;>>3#" """" ) ""' #"  ""&""" "" "( "  """#%"&&" "2""""'"+2 83.'.%1'"'&%2;;'2832;8';-33+".')+3'--883;;81&-82--22++"""), &%&2#''38#888,3332>3%38338'  * 8.H)9#I#%d35;R7HE41  .B9$.E BY+@%`:$U^-3bA 3YH0+,1>F /7Y<%[&8 Z&;75TD_@@d+"*Q4W972*`9_:TD= _#8QF-+-)M IOG *0<9 :TK)T00F E".I09(VK)&*T?1aCT4@@`6K77AW %AS8")*J/!> S*@>_=#L/(^I[QN?X:J Z<A+T\7L *(MT?:$4MCU"<ZZ2 \.5)+ 3&J %M%)85M PS#CZ.J(^@EaQD=:K- #1@9QU[&FWG> ,1d0(4 ($cEL/;2JZ\[CH0H "D" :L7;Q;9!"+,P H5/02TT.`9:O\kx?*CVN~]w&VEMq0/ vq<gR,G?<hDd-'7>m)]m.7F73 pRh%)]U>rZ06u/X kX8#u0(cxvFh[O{ nqT& "BG$W7rCHLtQR1T ylUizql[7\]+d9>ZbTJQUf@8FHx2 fr;LD1c02\A#{1  /wH`,z|'Ua :?6}A!i<,n_yjp~jsgDXtoj>C55U$F<XtB"'(9*'%713)(@$@SZ+#4; N %:8% !  ($ 6  0 @ @   @  @RSdp,4ppxIpyn i8oC~h@8ONUqnUcA,Z/~D 1.?.t$8Oy5U,Hx{QlT"iyCp~YJHPwtO?& u`CP;7fKHbO&-G(B`3v1>*a0q4,T=f[pw=?=d8xdiHT\xdw6v%JM~S=gF{B|wSfxJ@T| zG4n 60ae1=6 V=re;B_=U0g4Uv<1x}E9N9l]KKmGl/rqRU^gK`VeJ*tjWlb>z?f|Zy U4zM{Gf_{`k]cS8-;\1I\^ 84nVx@?E-CoS`kX'&3U]Z^]u@pej# Lfg!`PDW4+W'n|<4 :!Q9Ee6CKpnQzNktW_KFVcB~S9\h^koaDsQwsih}\fw&HgupV\v|D]CAAGCTCCTACTGGAGTTAAGGACAAAATAATAATTTGGGTGATGTTGCGCAACCCGTAACCGGCACTGAGATAACCGGTCTGTACATGCGGTGAGGGTGCAACCGGGGAGGATCTACATATGTGTAATAGCCACCATCTAGAACAGGTACACCTCAATTGTTAAAATATGGATCTTGCAGGTCAGTCGACTGAGCATCAGTCACACTATCTAAGGTGACGAAGCAACTCCGTTAGCTCTGCCCACTATTCACTGTCCTACAATCCGCCTTAATGGTATCGCCTACAGCATCGACTGGGGGTCTCTACTCCCTGGAATAGTGTCGCTAAGGTCATCGCTTGGGAAAATACCTCGGGGATCACGCCAGGTGAGAAGAGAAGAGTTTATGTTCTAGACTCTTCTCCATCTGAAGTAABCPFileName=KB.bcp;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=1;AverageSpacing=0;ProcessedDataProfile=1;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;KB 1.4.02010-08-05 17:18:41 +08:00AEPtAEPtAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSYBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'VDATA '~SDATA 'DATA '_DCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FWO_GATCGTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANE!LASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISP1AM>VP1RL>P1WD>P2AM>P2BANP2RL> PBASPBAS PCONyPCONPDMFVPDMFPLOC>PLOC>^PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%SCANASMED SMLtSMPLVSPACA^s=SPACSPACA^s=SVER3.0SVER PSVER,,2Scal@ScanATUBEH5Tmpr<UserMBphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPtAEPtAPFNMBAPXV2APrNMBAPrV9APrXww3ARTNASPFASPtASPtB1PtB1PtBCTSYBufT FMbCTIDMJCTNMMRCTOwMBCTTL 3CpEPDATAA4DATAA4!DATAA4UDATAA4DATA F DATA FDATA F IDATA F)DATA 'VDATA '~SDATA 'DATA '_DCHTDSamDySN 3lDye#DyeN3wDyeN3|DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3 EVNT3EVNT3,FTab#FVoc#FWO_GATCFeat//#tGTyp!!3KHCFGCEHCFGHCFGHCFG>>InScInVtLANE!LASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISP1AM>VP1RL>P1WD>P2AM>P2BANP2RL> PBASPBAS PCONyPCONPDMFVPDMFPLOC>PLOC>^PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%SCANASMED SMLtSMPLVSPACA^s=SPACSPACA^s=SVER3.0SVER PSVER,,2Scal@ScanATUBEH5Tmpr<UserMBphARphCHphDYbigphQLcphTRphTRTFo@L15@FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3k0(YS/F3&W,E)}Ns`AGqFQ9$5/qNE^O{?kJ:hAZG|JW>K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>Vt&J}<7B]%jyS*Y+3- l759Y6C}M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V.&h-[~!Tq*nEg8Z~d_1{63_iP-i2De! FXcXi-]js3W4KD@|Z`K'HACd}Fb[2yy>T\3>>B]'p8Npd-(bcn23i4Fbrtr%+6z={Z=p\E~rIG_3Th|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>Vl&od3m&?j|D-'Kx3} 6hf!Y '(doz1)U`]yVonIG}OKjm,P2t:Tj-ZZ]AE%AM&Z'K7rtlqaxkabC;Z6Obg`WpN}WQ`Q`e[lyo4$J_p[EwiOZ=kT/n@6TdO{7)-%TwKShKn6g;X6YMFBj'~;H28QQP-^ ppj0j!e~8LIG!Hgn_RJw JU6PX `HO5QfOl>o XS_9zH'zeE^O{U,Z:X=<;3On&+VDyK{t61h67A"yV+XZ>InScInVtLANE!LASTccLIMS!!3LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISP1AM>VP1RL>P1WD>P2AM>P2BANP2RL> PBASPBAS PCONyPCONPDMFVPDMFPLOC>PLOC>^PSZE`PTYPMZPXLBRGNmMBRGOwMBRMXV5RMdNE;RMdV5RMdXEJRPrNMBRPrV9RUND RUND RUND RUND RUNT RUNT RUNT 48RUNT Rate 3?RunNM+S/N%SCANASMED SMLtSMPL VSPACA^s=SPACSPACA^s=SVER3.0SVER PSVER,,2Scal@ScanATUBEH5Tmpr<UserMBphARphCHphDYbigphQLcphTRphTR